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KMID : 1059520080520060630
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Journal of the Korean Chemical Society
2008 Volume.52 No. 6 p.630 ~ p.636
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Rapid Distinction of Hanwoo and Imported beef by Single Strand Conformation Polymorphism-Capillary Electrophoresis
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Go Hye-Ah
Lee Mi-Ji
Li Song-Lan
Kim Kwan-Suk
Kang Seong-Ho
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Abstract
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We developed a rapid and simple method for distinguish of Hanwoo and imported beef by capillary electrophoresis based on the single strand conformation polymorphism(SSCP-CE). PCR amplification was performed to amplify specific DNA regions spanning single nucleotide polymorphism(SNP) markers to determine between Hanwoo and imported beef. The SSCP ss-DNA fragments from the amplified PCR products were analyzed through the fusedsilica capillary(75 μm I.D. × 365 μm O.D.; effective length, 30 cm; total length, 50 cm) filled with the mixed polymer of 2.2%(w/v) poly(ethyleneoxide)(Mr 600 000) and 1.0%(w/v) poly(ethyleneoxide)(Mr 8 000 000) as a sieving matrix. Under the electric fields of 360 V/cm, Hanwoo and imported beef were simply distinguished within only 13 min by SSCP-CE.
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KEYWORD
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Hanwoo, Capillary Electrophoresis, PCR, Single Strand Conformation Polymorphism
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